RNase E biomolecular condensates stimulate PNPase activity Michael J Collins, Dylan T Tomares, Vidhyadhar Nandana, Jared M Schrader, W Seth Childers Scientific Reports 2023, 13, 12937
Scaffold-Scaffold Interaction Facilitates Cell Polarity Development in Caulobacter crescentus Ning Lu, Samuel W Duvall, Guohong Zhao, Kimberley A Kowallis, Chao Zhang, Wei Tan, Jingxian Sun, Haley N Petitjean, Dylan T Tomares, Guo-Ping Zhao, W Seth Childers, Wei Zhao mBio 2023, 14, e03218-22
Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub,” Wei Tan, Sihua Cheng, Yingying Li, Xiao-Yang Li, Ning Lu, Jingxian Sun, Guiyue Tang, Yujiao Yang, Kezhu Cai, Xuefei Li, Xijun Ou, Xiang Gao, Guo-Ping Zhao, W Seth Childers, Wei Zhao Nature Communications 2022, 13, 7181
“Repurposing peptide nanomaterials as synthetic biomolecular condensates in bacteria,” Dylan T. Tomares, Sara Whitlock, Matthew Mann, Emma DiBernardo, W. Seth Childers ACS Synthetic Biology 2022, 11, 6, 2154–2162
“Regulation of a the activity of the bacterial histidine kinase PleC by the scaffold protein PodJ ,” Chao Zhang, Wei Zhao, Samuel W. Duvall, Kimberley A. Kowallis and W. Seth Childers Journal of Biological Chemistry 2022, 298 101683
“The Future Potential of Biosensors to investigate the brain-gut-axis" Jiefei Wang and W. Seth Childers Frontiers in Bioengineering and Biotechnology 2022
Protein engineering strategies to study bacterial pseudokinases Yang, X., Kowallis, K.A., Childers, W.S.Methods of Enzymology 2022
“A Biosensor for the detection of indole metabolities ,” Jiefei Wang#, Chao Zhang# and W. Seth Childers ACS Synthetic Biology 2021.
“The Upcycled Roles of Pseudoenyzmes in two-component signal transduction ,” Michael Collins and W. Seth Childers, Current Opinion in Microbiology, 2021.
“Synthetic Control of Signal Flow Within a Bacterial Multi-Kinase Network,” Kimberley A Kowallis, Elayna M. Silfani, Amanda Kasumu, Grace Rong, Victor So, and W. Seth Childers, ACS Synthetic Biology, 2020.
“Design of a Histidine Kinase FRET Sensor to Detect Complex Signal Integration within Living Bacteria,” Samuel W. Duvall, W. Seth Childers, ACS Sensor, 2020.
“Phase-separated bacterial ribonucleoprotein bodies organize mRNA decay,” Nisansala S. Muthunayake, Dylan T. Tomares, W. Seth Childers, Jared M. Schrader, WIREs RNA, 2020.
“BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates,” Nadra Al-Husini, Dylan T. Tomares, Zechariah Pfaffenberger, Nisansala S. Muthunayake, Mohammad A. Samad, Tiancheng Zuo, Obaidah Bitar, James R. Aretakis, Mohammed-Husain M. Bharmal, Alisa Gega, Julie S. Biteen, W. Seth Childers*, Jared M. Schrader*, Molecular Cell, 2020. *corresponding authors
"Manipulation of bacterial signaling using engineered histidine kinases.” in Histidine Phosphorylation: Methods and Protocols. Kimberly A Kowallis, Samuel W. Duvall, Wei Zhao, W. Seth Childers, Springer Protocols, 2019
“α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies,” Nadra Al-Husini, Dylan T. Tomares, Obaidah Bitar, W. Seth Childers*, & Jared M. Schrader*. Mol Cell, 2018 *corresponding authors
Publications:
“Repurposing peptide nanomaterials as synthetic biomolecular condensates in bacteria,” Dylan T. Tomares, Sara Whitlock, Matthew Mann, Emma DiBernardo, W. Seth Childers ACS Synthetic Biology 2022 In press
“Regulation of a the activity of the bacterial histidine kinase PleC by the scaffold protein PodJ ,” Chao Zhang, Wei Zhao, Samuel W. Duvall, Kimberley A. Kowallis and W. Seth Childers Journal of Biological Chemistry 2022
“The Future Potential of Biosensors to investigate the brain-gut-axis" Jiefei Wang and W. Seth Childers Frontiers in Bioengineering and Biotechnology 2022
“A Biosensor for the detection of indole metabolities ,” Jiefei Wang#, Chao Zhang# and W. Seth Childers ACS Synthetic Biology 2021.
“The Upcycled Roles of Pseudoenyzmes in two-component signal transduction ,” Michael Collins and W. Seth Childers, Current Opinion in Microbiology, 2021.
“Synthetic Control of Signal Flow Within a Bacterial Multi-Kinase Network,” Kimberley A Kowallis, Elayna M. Silfani, Amanda Kasumu, Grace Rong, Victor So, and W. Seth Childers, ACS Synthetic Biology, 2020.
“Design of a Histidine Kinase FRET Sensor to Detect Complex Signal Integration within Living Bacteria,” Samuel W. Duvall, W. Seth Childers, ACS Sensor, 2020.
“Phase-separated bacterial ribonucleoprotein bodies organize mRNA decay,” Nisansala S. Muthunayake, Dylan T. Tomares, W. Seth Childers, Jared M. Schrader, WIREs RNA, 2020.
“BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates,” Nadra Al-Husini, Dylan T. Tomares, Zechariah Pfaffenberger, Nisansala S. Muthunayake, Mohammad A. Samad, Tiancheng Zuo, Obaidah Bitar, James R. Aretakis, Mohammed-Husain M. Bharmal, Alisa Gega, Julie S. Biteen, W. Seth Childers, Jared M. Schrader, Molecular Cell, 2020.
"Manipulation of bacterial signaling using engineered histidine kinases.” in Histidine Phosphorylation: Methods and Protocols. Kimberly A Kowallis, Samuel W. Duvall, Wei Zhao, W. Seth Childers, Springer Protocols, 2019
“α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies,” Nadra Al-Husini, Dylan T. Tomares, Obaidah Bitar, W. Seth Childers, & Jared M. Schrader. Mol Cell, 2018
Publications with Lucy Shapiro at Stanford University
"Dynamic translation regulation in Caulobacter cell cycle control," Schrader, J.M., Li, G.W., Childers, W.S., Perez, A.M., Weissman, J.S., Shapiro, L. & McAdams, H.H., Proc Natl Acad Sci., 2016
"A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues," Mann, Thomas H., Seth Childers, W., Blair, Jimmy A., Eckart, Michael R. & Shapiro, L., Nat. Commun., Vol. 2, 2016, Pages 1-12
“A pseudokinase couples signaling pathways to enable asymmetric cell division in a bacterium,” Childers, W.S., Shapiro L., Microbial Cell, 2014, Pages 29-32
“Cell Fate Regulation Governed by a Repurposed Bacterial Histidine Kinase,” Childers, W.S., Xu, Q., Mann, T.H., Irimpan I.M., Blair, J.A., Deacon, A.M., Shapiro L., PLOS Biology, 2014, e1001979
“Branched Signal Wiring of an Essential Bacterial Cell-Cycle Phosphotransfer Protein,” Blair, J.A.*, Xu, Q.*, Childers, W.S.*, Irimpan I.M, Kern J.W., Eckart M. Deacon, A.M., Shapiro L.,Structure, Vol. 21, 2013, Pages 1590-1601
Publications with David Lynn at Emory University
“Phase Networks of Cross-β assemblies,” Childers, W.S., Anthony, N.R., Berland, K.M., Mehta, A.K. & Lynn, D.G., Langmuir, Vol. 28, 2012, Pages 6386-6395
“Remodeling Cross-β Nanotube Surfaces with Peptide/Lipid Chimeras,” Ni, R., Childers, W.S., Hardcastle, K.I., Mehta, A.K. & Lynn, D.G., Angewandte Chemie Int Ed., Vol. 124, 2012, Pages 6739-6742
“Towards intelligent materials. Molecular Self-Assembly Advances and Applications. 1st ed.,” Childers, W.S., Mehta, A.K. & Lynn, D.G., Pan Stanford Publishing Pte Ltd: Singapore/SG, 2012,Pages 1-36
“Peptides organized as bilayer membranes,” Childers, W.S., Mehta, A.K., Ni, R., & Lynn, D.G.,Angewandte Chemie Int Ed., Vol. 49, 2010, Pages 4104-4107
“Peptide membranes in chemical evolution,” Childers, W.S, Ni, R., Mehta, A.K., & Lynn, D.G.,Current Opinion Chemical Biology., Vol. 13, 2010, Pages 652-659
“Templating molecular arrays in amyloid’s cross-β grooves,” Childers, W.S, Mehta, A.K., & Lynn, D.G., J Am Chem Soc., Vol. 131, 2009, Pages 10165-10172
“Facial symmetry in protein self-assembly,” Mehta, A.K., Lu, K., Childers, W.S, & Lynn, D.G., J Am Chem Soc., Vol. 130, 2007, Pages 9829-9835
“Engineering metal ion coordination to regulate amyloid fibril assembly and toxicity,” Dong, J., Canfield, J.M., Mehta, A.K., Shokes, J.E., Tian, B., Childers, W.S, Simmons, J.A., Mao, Z., Scott, R., Warncke, K., & Lynn, D.G., Proc Natl Acad Sci., Vol. 104, 2007, Pages 13313-13318
“Repurposing peptide nanomaterials as synthetic biomolecular condensates in bacteria,” Dylan T. Tomares, Sara Whitlock, Matthew Mann, Emma DiBernardo, W. Seth Childers ACS Synthetic Biology 2022 In press
“Regulation of a the activity of the bacterial histidine kinase PleC by the scaffold protein PodJ ,” Chao Zhang, Wei Zhao, Samuel W. Duvall, Kimberley A. Kowallis and W. Seth Childers Journal of Biological Chemistry 2022
“The Future Potential of Biosensors to investigate the brain-gut-axis" Jiefei Wang and W. Seth Childers Frontiers in Bioengineering and Biotechnology 2022
“A Biosensor for the detection of indole metabolities ,” Jiefei Wang#, Chao Zhang# and W. Seth Childers ACS Synthetic Biology 2021.
“The Upcycled Roles of Pseudoenyzmes in two-component signal transduction ,” Michael Collins and W. Seth Childers, Current Opinion in Microbiology, 2021.
“Synthetic Control of Signal Flow Within a Bacterial Multi-Kinase Network,” Kimberley A Kowallis, Elayna M. Silfani, Amanda Kasumu, Grace Rong, Victor So, and W. Seth Childers, ACS Synthetic Biology, 2020.
“Design of a Histidine Kinase FRET Sensor to Detect Complex Signal Integration within Living Bacteria,” Samuel W. Duvall, W. Seth Childers, ACS Sensor, 2020.
“Phase-separated bacterial ribonucleoprotein bodies organize mRNA decay,” Nisansala S. Muthunayake, Dylan T. Tomares, W. Seth Childers, Jared M. Schrader, WIREs RNA, 2020.
“BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates,” Nadra Al-Husini, Dylan T. Tomares, Zechariah Pfaffenberger, Nisansala S. Muthunayake, Mohammad A. Samad, Tiancheng Zuo, Obaidah Bitar, James R. Aretakis, Mohammed-Husain M. Bharmal, Alisa Gega, Julie S. Biteen, W. Seth Childers, Jared M. Schrader, Molecular Cell, 2020.
"Manipulation of bacterial signaling using engineered histidine kinases.” in Histidine Phosphorylation: Methods and Protocols. Kimberly A Kowallis, Samuel W. Duvall, Wei Zhao, W. Seth Childers, Springer Protocols, 2019
“α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies,” Nadra Al-Husini, Dylan T. Tomares, Obaidah Bitar, W. Seth Childers, & Jared M. Schrader. Mol Cell, 2018
Publications with Lucy Shapiro at Stanford University
"Dynamic translation regulation in Caulobacter cell cycle control," Schrader, J.M., Li, G.W., Childers, W.S., Perez, A.M., Weissman, J.S., Shapiro, L. & McAdams, H.H., Proc Natl Acad Sci., 2016
"A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues," Mann, Thomas H., Seth Childers, W., Blair, Jimmy A., Eckart, Michael R. & Shapiro, L., Nat. Commun., Vol. 2, 2016, Pages 1-12
“A pseudokinase couples signaling pathways to enable asymmetric cell division in a bacterium,” Childers, W.S., Shapiro L., Microbial Cell, 2014, Pages 29-32
“Cell Fate Regulation Governed by a Repurposed Bacterial Histidine Kinase,” Childers, W.S., Xu, Q., Mann, T.H., Irimpan I.M., Blair, J.A., Deacon, A.M., Shapiro L., PLOS Biology, 2014, e1001979
“Branched Signal Wiring of an Essential Bacterial Cell-Cycle Phosphotransfer Protein,” Blair, J.A.*, Xu, Q.*, Childers, W.S.*, Irimpan I.M, Kern J.W., Eckart M. Deacon, A.M., Shapiro L.,Structure, Vol. 21, 2013, Pages 1590-1601
Publications with David Lynn at Emory University
“Phase Networks of Cross-β assemblies,” Childers, W.S., Anthony, N.R., Berland, K.M., Mehta, A.K. & Lynn, D.G., Langmuir, Vol. 28, 2012, Pages 6386-6395
“Remodeling Cross-β Nanotube Surfaces with Peptide/Lipid Chimeras,” Ni, R., Childers, W.S., Hardcastle, K.I., Mehta, A.K. & Lynn, D.G., Angewandte Chemie Int Ed., Vol. 124, 2012, Pages 6739-6742
“Towards intelligent materials. Molecular Self-Assembly Advances and Applications. 1st ed.,” Childers, W.S., Mehta, A.K. & Lynn, D.G., Pan Stanford Publishing Pte Ltd: Singapore/SG, 2012,Pages 1-36
“Peptides organized as bilayer membranes,” Childers, W.S., Mehta, A.K., Ni, R., & Lynn, D.G.,Angewandte Chemie Int Ed., Vol. 49, 2010, Pages 4104-4107
“Peptide membranes in chemical evolution,” Childers, W.S, Ni, R., Mehta, A.K., & Lynn, D.G.,Current Opinion Chemical Biology., Vol. 13, 2010, Pages 652-659
“Templating molecular arrays in amyloid’s cross-β grooves,” Childers, W.S, Mehta, A.K., & Lynn, D.G., J Am Chem Soc., Vol. 131, 2009, Pages 10165-10172
“Facial symmetry in protein self-assembly,” Mehta, A.K., Lu, K., Childers, W.S, & Lynn, D.G., J Am Chem Soc., Vol. 130, 2007, Pages 9829-9835
“Engineering metal ion coordination to regulate amyloid fibril assembly and toxicity,” Dong, J., Canfield, J.M., Mehta, A.K., Shokes, J.E., Tian, B., Childers, W.S, Simmons, J.A., Mao, Z., Scott, R., Warncke, K., & Lynn, D.G., Proc Natl Acad Sci., Vol. 104, 2007, Pages 13313-13318